Team:TU Darmstadt/protocols/PCR

Short report
PCR (polymerase chain reaction) is a method for exponentially amplifying a fragment of DNA in vitro. Materials

Equipment
Micropipettes with sterile tips
PCR machine
PCR tubes

Chemicals & consumables
DNA template
Nuclease-free water
Primer
Polymerase buffer
dNTP mix
DMSO

Procedure
This is a commonly used protocol thta we used for Taq-polymerase
PCR program (30 cycles)
initial denaturation 95°C, 60s
denaturation 95°C, 45s
annealing 54,5°C, 90s
elongation 72°C, 120s
final elongation 72°C, 120s

After worth run an analytic 1% agarose gel and purificate the product with Wizard SV Gel and PCR Clean-Up System (Promega)